EC Number   |
Protein Variants |
Reference  |
|---|
    2.2.1.6 | more |
mutants with overexpression of 2-acetolactate synthase (ALS), 2-acetolactate decarboxylase, and acetoin reductase, either individually or in combination, are constructed to improve 2,3-butanediol production in Klebsiella pneumoniae. The strain (KG-rs) that overexpresses both 2-acetolactate synthase and acetoin reductase shows an improved 2,3-butanediol yield. When cultured in the media with five different carbon sources (glucose, galactose, fructose, sucrose, and lactose), the mutant exhibits higher 2,3-butanediol productivity and production than the parental strain in all the tested carbon sources except for lactose. The 2,3-butanediol production of strain KG-rs in a batch fermentation with glucose as the carbon source is 12% higher than that of the parental strain, overview |
-, 733547 |
| 2.2.1.6 | A122V |
reduced affinity for all Mg2+, thiamine diphosphate and FAD |
395903 |
| 2.2.1.6 | S653N |
binds FAD more strongly than the wild-type enzyme |
395903 |
| 2.2.1.6 | W574L |
insensitive to sulfonurea herbicides |
395903 |
| 2.2.1.6 | W574S |
reduction in sensitivity to sulfonurea herbicides compared to the wild-type enzyme |
395903 |
| 2.2.1.6 | G16D/E105stop |
no enzymic activity after in vitro reconstitution with large subunit |
657717 |
| 2.2.1.6 | I106V/A135P |
after in vitro reconstitution with large subunit, enzymic activity comparable to wild-type |
657717 |
| 2.2.1.6 | L18F |
no enzymic activity after in vitro reconstitution with large subunit |
657717 |
| 2.2.1.6 | Q108stop |
no enzymic activity after in vitro reconstitution with large subunit |
657717 |
| 2.2.1.6 | V17D |
no enzymic activity after in vitro reconstitution with large subunit |
657717 |
