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Results 1 - 10 of 295 > >>
EC Number
Amino acid exchange
Commentary
Reference
C173A/S174A
modest defect, grows well at 19, 30 and 34C, but very slowly at 37C
C382Y
15-50% of wild-type activity
C382Y
72% of wild-type methylation activity
C414R
complete loss of methylation activity
C414R
mutation abolishes the N7-MTase activity on GTP
C416R
mutation abolishes the N7-MTase activity on GTP
D122A/S123A
site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25C and 30C, only slightly at 37C
D144A
TbCmt1 mutant shows 5% of wild-type activity
D146A
mutant completely loses the N-7 activity (methylation of N-7 position of guanine) and 2'-O methylation activity. Mutation defective in N-7 methylation is lethal for DNV replication
D192A/S193A
temperature-sensitive vD12 allele, near-lethality, only grows very slowly at 19C
Results 1 - 10 of 295 > >>