EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.274 | M156H |
site-directed mutagenesis, the mutation of Met156 to His results in a switch from a preference for salicylic acid (SA) over benzoic acid (BA) in wild-type PtSABATH4 to a preference for BA over SA in the M156H mutant |
757984 |
| 2.1.1.274 | M314V |
site-directed mutagenesis, the mutation results in decreased enzymatic activities towards both the substrates salicylic acid (SA) over benzoic acid (BA), but not in a substrate switch |
757984 |
| 2.1.1.274 | more |
an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout |
-, 757709 |
| 2.1.1.274 | more |
silencing of gene NbSAMT, quantitative real-time PCR defense-related genes expression analysis |
736841 |
| 2.1.1.274 | Y147S |
reduced methylation of benzoic acid and 3-hydroxybenzoic acid but slightly increased activity towards jasmonic acid |
723402 |
| 2.1.1.274 | Y147S/M150H |
significant increase in the ability to turn over jasmonic acid and vanillic acid |
723402 |
| 2.1.1.274 | Y147S/M150H/F347Y |
slightly increased activity towards short-chain carboxylic acids and jasmonic acid |
723402 |
| 2.1.1.274 | Y147S/M150H/F347Y/N349I |
highly increased activity towards short-chain carboxylic acids and aromatic acids like hydroxybenzoic acids, vanillic acid, jasmonic acid, cinnamic acid, 4-coumaric acid and caffeic acid, broadest substrate spectrum |
723402 |
| 2.1.1.274 | Y147S/M150H/I225Q/F347Y |
greatest specific activities against 3-hydroxybenzoic acid, vanillic acid and jasmonic acid but reduced activity towards salicylic acid |
723402 |
