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Amino acid exchange
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an engineered strain named Aspergillus nidulans WFC, expressing ergot alkaloid synthesis genes dmaW, easF, and easC, produces the established intermediate N-methyl-4-dimethylallyltryptophan, as well as an ergot alkaloid, that is characterized as ergotryptamine, it differs from N-methyl-4-dimethylallyltryptophan by the loss of the carboxyl group, addition of a hydroxyl group, and shift in position of a carbon-carbon double bond, by mass spectrometry, labeling, and NMR. Feeding studies with Aspergillus mutants do not show ergotryptamine turnover, suggesting it is a pathway byproduct as opposed to an authentic intermediate
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