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Results 1 - 8 of 8
EC Number
Amino acid exchange
Commentary
Reference
C168A
site-directed mutagenesis, the mutation does not affect KsgA activity
E43A
mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature
E66A
catalytically inactive enzyme form
E66A
mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature
G47A
mutation located in the S-adenosylmethionine-binding motifs severely reduces methyltransferase activity, the mutation retains the ability to suppress the growth defect of the Era(E200K) strain at a low temperature
L114P
site-directed mutagenesis of the active site residue, the KsgA mutant shows diminished overall activity, and impaired ability to methylate the N6-methyladenosine intermediate to produce N6,N6-dimethyladenosine. Reduced activity is not due to disruption of 30S substrate binding
N113A
site-directed mutagenesis of the active site residue, the KsgA mutant shows diminishes activity to a level comparable to L114P without affecting the methylation of N6-methyladenosine. Reduced activity is not due to disruption of 30S substrate binding
R248A
mutation at the C-terminal does not affect the methyltransferase activity and fails to suppress the growth defect of the Era(E200K) strain
Results 1 - 8 of 8