EC Number |
Protein Variants |
Reference |
---|
2.1.1.158 | more |
construction of several enzyme mutants, overview. The mutants of CmXRS1, that have 3-N methylation activity and produce caffeine from paraxanthine as a substrate, need to have replacement of the glutamine residue by histidine at position 161 in the CmXRS1 sequence, i.e. a Q161H mutation, overview |
721124 |
2.1.1.158 | more |
reduction of the second enzyme of the pathway, 7-methylxanthine methyltransferase, EC 2.1.1.159, leads to reduced XMT1 expression |
670565 |
2.1.1.158 | A23S/P104Q/Q161H/H219R |
site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows 60% of wild-type 7-methylation activity |
721124 |
2.1.1.158 | A23S/P104Q/Q161H/L191P/H219R |
site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity |
721124 |
2.1.1.158 | A23S/Q161H/H219R |
site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity |
721124 |
2.1.1.158 | A23S/Q161H/L191P/H219R |
site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity |
721124 |
2.1.1.158 | P104Q/Q161H |
site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity |
721124 |
2.1.1.158 | P104Q/Q161H/L191P |
site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity |
721124 |
2.1.1.158 | Q161H |
site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity |
721124 |
2.1.1.158 | Q161H/L191P |
site-directed mutagenesis, the mutant exhibits 3-N methylation activity in contrast to the wild-type enzyme, the mutant shows over 80% of wild-type 7-methylation activity |
721124 |