EC Number   |
Protein Variants |
Reference |
|---|
    2.1.1.14 | N126A |
13% of wild-type activity |
-, 734490 |
| 2.1.1.14 | Y660Q |
14% of wild-type activity |
-, 734490 |
| 2.1.1.14 | Y660F |
93% of wild-type activity |
-, 734490 |
| 2.1.1.14 | V39A/R46C/T106I/K713E/C645A |
C645A mutation additionally improves acetate tolerance. Strains harboring acetate-tolerant MetE mutants are less inhibited by homocysteine in L-isoleucine-enriched medium. The acetate-tolerant MetE mutants stimulate the growth of the host strain at elevated temperatures of 44 and 45°C. The mutant MetE enzymes display a reduced melting temperature but an enhanced in vivo stability |
733170 |
| 2.1.1.14 | V39A/R46C/T106I/K713E |
mutant confers accelerated growth in the Escherichia coli K-12 WE strain in the presence of acetate. Strains harboring acetate-tolerant MetE mutants are less inhibited by homocysteine in L-isoleucine-enriched medium. The acetate-tolerant MetE mutants stimulate the growth of the host strain at elevated temperatures of 44 and 45°C. The mutant MetE enzymes display a reduced melting temperature but an enhanced in vivo stability |
733170 |
| 2.1.1.14 | C726S |
mutant does not contain zinc, no activity, probably due to lack of zinc |
441332 |
| 2.1.1.14 | D113R |
mutation abrogate nuclear localization |
757179 |
| 2.1.1.14 | Y660A |
no residual activity |
-, 734490 |
| 2.1.1.14 | R742A |
nuclear localization is abrogated by the deletion of 107 C-terminal amino acids or the R742A mutation |
757179 |
| 2.1.1.14 | C645A |
the mutation confers resistance to diamide when cells are grown in media lacking methionine, but not when cells are grown in the presence of methionine, cysteine 645 serves to modulate the activity of MetE in vivo in response to disulfide stress |
704294 |
