EC Number |
Protein Variants |
Reference |
---|
1.7.1.13 | C190A |
complete loss of activity |
724767 |
1.7.1.13 | C190A |
mutation in catalytic residue, no evidence of covalent binding of substrate preQ0. Mutant displays proton uptake |
742900 |
1.7.1.13 | C190A |
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity |
764418 |
1.7.1.13 | C190S |
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity |
764418 |
1.7.1.13 | C99A |
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible |
-, 742061 |
1.7.1.13 | C99S |
mutant does not compromise enzyme activity. Contrary to wild-type, peroxide-induced inactivation is irreversible |
-, 742061 |
1.7.1.13 | D197A |
the variant does not take up protons in conjunction with preQ0 binding |
764418 |
1.7.1.13 | D197H |
the variant recovers proton uptake to a level almost analogous to that seen with the wildtype enzyme |
764418 |
1.7.1.13 | D197N |
complete loss of activity |
724767 |
1.7.1.13 | E230Q |
about 4% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one |
724767 |