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EC Number
Amino acid exchange
Commentary
Reference
D370A
mutant form of PutA86-601. The ratio of turnover-number to Km-value is 2.8fold lower than the ratio of the wild-type PutA86-601
D370A/Y540F
mutant form of PutA86-601. The ratio of turnover-number to Km-value is 15fold lower than the ratio of the wild-type PutA86-601
F10E/L12E
construction of a variant with a more polar N-terminus, mutant F10E/L12E, because Thermus thermophilus ProDH (TtProDH), produced through fusion with maltose-binding protein (MBP) appears to be prone to aggregation. Preparation of TtProDH mutant variant DELTAABC, which lacks helices alphaA, alphaB and alphaC, the mutant shows no electron density for an AMP moiety of the cofactor
K110A
inactive
L432P
mutant form of PutA86-669, 5fold decrease in turnover-number and a severe loss in thermostability
more
expression of PRODH/POX in DLD-1 colorectal cancer cells significantly decreases basal and maximal respiration at both 3 and 5 days, and proline addition exacerbates this effect. PRODH/POX-dependent inhibition of respiration can be modulated by the duration of PRODH/POX expression and the availability of proline
more
generation of an enzyme-deficient prodh1 mutant
more
mutants deficient in proline dehydrogenase activity
more
the truncated form of Pseudomonas putida PutA shows proline dehydrogenase activity
more
truncated enzyme containing residues 86-601 and only four Trp residues. Substantial conformational changes of truncated protein upon addition of proline
Results 1 - 10 of 17 > >>