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Results 1 - 5 of 5
EC Number
Amino acid exchange
Commentary
Reference
C47A
beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme
C50A
beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme
C55A
beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme
C59A
beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme
G298A
mutant with an approximately 10fold decrease of the affinity of the enzyme for pyridine nucleotides with little or no effect on the rate of the enzyme reduction by NADPH, maintains the ability to bind NADPH and FAD, is catalytically active in the NADPH:iodonitrotetrazolium oxidoreductase reaction and has a monomeric state, mutation leads to production of insoluble protein under conditions that yield large amounts of soluble wild-type enzyme and to production of smaller amounts of enzyme
Results 1 - 5 of 5