EC Number   |
Protein Variants |
Reference  |
|---|
    1.3.98.3 | C62S |
inactive mutant, no Fe-S cluster formation |
656073 |
| 1.3.98.3 | C66S |
inactive mutant, no Fe-S cluster formation |
656073 |
| 1.3.98.3 | C69S |
inactive mutant, no Fe-S cluster formation |
656073 |
| 1.3.98.3 | C71S |
inactive mutant, same Fe-S cluster formation as in wild-type HemN |
656073 |
| 1.3.98.3 | F68L |
mutant with 89% of wild-type activity |
656073 |
| 1.3.98.3 | G111V/G113V |
double mutation of Gly-111 and Gly-113, which are part of the potential GGGTP S-adenosyl-L-methionine binding motif, completely abolishes enzyme activity, reduced Fe-S cluster formation |
656073 |
| 1.3.98.3 | H58L |
inactive mutant, no Fe-S cluster formation |
656073 |
| 1.3.98.3 | Y56F |
mutant with 45% of wild-type activity and reduced Fe-S cluster formation |
656073 |
| 1.3.98.3 | E145A |
appears colorless, the [4Fe-4S] cluster content is slightly reduced, no detectable S-adenosylmethionine cleavage, no detectable CPO activity |
674462 |
| 1.3.98.3 | E145I |
appears colorless, the [4Fe-4S] cluster content is slightly reduced, no detectable S-adenosylmethionine cleavage, no detectable CPO activity |
674462 |
