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EC Number
Amino acid exchange
Commentary
Reference
S175A
-
R265A
2fold increase in KM-value of CoQD, 15% increase in KM-value of L-dihydroorotate
H185A
4fold increase in KM-value of CoQD, 50% increase in KM-value of L-dihydroorotate
L71F/C130S/V133T
addition of the two residues comprising the conserved proton-transfer network of Class 2 dihydroorotate dehydrogenase from Escherichia coli to the C130S Class 1A enzyme of Lactococcus lacits. Mutation does not did not restore the function of the active site base or rapid flavin reduction. Kd for dihydroorotate is about three times tighter than the wild-type
E206/K296E
conversion of intermolecular salt bridge, mutant is fully active in concentrated solutions and dissociates into monomers upon dilution like wild-type enzyme
K213E
decrease in activity against dichlorophenolindophenol
P131A
decrease in activtiy against fumarate
S129A
decrease in activtiy against fumarate
more
deletions of N-terminal residues from 2 down to 40 result in generally unstable proteins with apo protein quickly precipitating and FMN remaining in solution. A truncated protein lacking residues 2 to 30 is sufficiently stable, has near to wild-type activity using molecular oxygen and 20fold lower activity using 2,6-dichlorophenolindophenol as electron acceptor
more
DHOD-knockout Trypanosoma cruzi do not express the enzyme protein and can not survive even in the presence of pyrimidine nucleosides, suggesting a vital role of fumarate reductase activity in the regulation of cellular redox balance
Results 1 - 10 of 31 > >>