Refine search

Search Engineering

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 10 of 31 > >>
EC Number
Amino acid exchange
Commentary
Reference
C130A
low activity with dihydroorotate, increasing activity above pH 8.0 with dihydrooxonate
C130A
the mutant enzyme exhibits binding affinities for dihydroorotate similar to that of the wild type enzyme, reduction is extremely slow compared to that of the wild type, the rate of reduction increases with pH showing no sign of a plateau
C130A
the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 590 nm
C130S
the mutant enzyme exhibits binding affinities for dihydroorotate similar to that of the wild type enzyme, reduction is extremely slow compared to that of the wild type, the rate of reduction increases with pH showing no sign of a plateau
C130S
the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 610 nm
E206/K296E
conversion of intermolecular salt bridge, mutant is fully active in concentrated solutions and dissociates into monomers upon dilution like wild-type enzyme
E206A
disturbance of intermolecular salt bridge, mutant retains almost complete activity
E206K
disturbance of intermolecular salt bridge, mutant activity is severely impaired
H185A
4fold increase in KM-value of CoQD, 50% increase in KM-value of L-dihydroorotate
K136E
little changes in activity
Results 1 - 10 of 31 > >>