EC Number   |
Protein Variants |
Reference |
|---|
   1.3.7.4 | more |
identification of elongated hypocotyl-1 (elh1) mutant C1238, comparison of mutant seedlings with wild-type CCMC seedlings, phenotype, overview. Analysis of the effect of CsHY2 mutation on the function of phys, the expression dynamics of cucumber PHYs genes (CsPHYA1, CsPHYA2, CsPHYB, CsPHYC, and CsPHYE). The 35S:CsHY2-EGFP plasmid construct is transformed into Agrobacterium tumefaciens strain GV3101. Arabidopsis hy2-1 recessive homozygous mutants are transformed and the phenotype is analyzed. The expression level of CsHY2 in mutant is the highest in male and female flowers, followed in order by stem, true leaves, root, cotyledon, and fruit. There is no significant difference in CsHY2 expression in all these organs except cotyledons, true leaves, and stem between wild-type and mutant elh1 |
763754 |
| 1.3.7.4 | D146N |
mutant completely loses catalytic activity and also the ability of biliverdin binding |
687760 |
| 1.3.7.4 | R252Q |
mutant loses catalytic activity and the ability of substrate binding |
687760 |
| 1.3.7.4 | N133 |
mutant produces only partial activity |
687760 |
| 1.3.7.4 | D256E |
mutant retains only partial activity |
687760 |
| 1.3.7.4 | D116N |
mutant still retains the ability of substrate binding, but with only 1.5% relative activity of wild type protein |
687760 |
| 1.3.7.4 | V121A |
site-directed mutagenesis, single-turnover analysis demonstrates that the V121A mutated protein is slightly slower, although it produces 3Z/E-PPhiB on wild-type level, whereas no activity is detected in the V121A mutated protein in the steady-state analysis |
763272 |
| 1.3.7.4 | K255Q |
site-directed mutagenesis, the mutant shows 11.7% of wild-type activity |
712484 |
| 1.3.7.4 | R200Q/R264Q |
site-directed mutagenesis, the mutant shows 11.9% of wild-type activity |
712484 |
| 1.3.7.4 | R200Q |
site-directed mutagenesis, the mutant shows 12.5% of wild-type activity |
712484 |
