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Results 1 - 6 of 6
EC Number
Amino acid exchange
Commentary
Reference
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a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol
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construction of a a hybrid gene consisting of the 5'half of Rhodospirillum rubrum bchP and the 3' half of Rhodobacter sphaeroides bchP, with the fragments fused as for bchPDELTAx, possesses neither geranylgeranyl-bacteriopheophytin reductase nor geranylgeranyl-bacteriochlorophyll a reductase activity. When complenmentation of the mutant is attempted with a similar fusion between the 5' half of Rhodobacter sphaeroides bchP and the 3' half of Rhodospirillum rubrum bchP, expressed in plasmid pSK1bchPS/PR, not only is BpheaP biosynthesis restored, but approximately 10% of the Bchl present is found to be esterified with dihydro-GG. Complete restoration of both BchlaP and BpheaP synthesis is achieved by using the positive control plasmid, pSK1bchPS/PS, in which the two relevant Rhodobacter sphaeroides bchP fragments are reunited
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construction of a chromosomal KmR insertion mutation of gene bchP performed by GTA-mediated homologous recombination
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construction of a hybrid gene consisting of the 5' half of Rhodospirillum rubrum bchP and the 3' half of Rhodobacter sphaeroides bchP, with the fragments fused as for bchPDELTAx, possesses neither geranylgeranyl-bacteriopheophytin reductase nor geranylgeranyl-bacteriochlorophyll a reductase activity. When complenmentation of the mutant is attempted with a similar fusion between the 5' half of Rhodobacter sphaeroides bchP and the 3' half of Rhodospirillum rubrum bchP, expressed in plasmid pSK1bchPS/PR, not only is BpheaP biosynthesis restored, but approximately 10% of the Bchl present is found to be esterified with dihydro-GG. Complete restoration of both BchlaP and BpheaP synthesis is achieved by using the positive control plasmid, pSK1bchPS/PS, in which the two relevant Rhodobacter sphaeroides bchP fragments are reunited
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generation of a gene CT2256 deletion mutant, which almost shows no apparent phenotype compared to the wild-type. The purple bacterium Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene is partially complemented with the CT2256 gene, bacteriochlorophyllide a is synthesized in the mutant in addition to accumulating other intermediates, phenotype, overview
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the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue encoded by gene chlP, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, phenotype, overview
Results 1 - 6 of 6