EC Number |
Protein Variants |
Reference |
---|
1.20.1.1 | A146S |
increases the half-life of thermal inactivation at 45°C from around 1 min to 8 min |
691424 |
1.20.1.1 | A176R |
strongly decreased Km for NADP+ |
657956 |
1.20.1.1 | A319E |
increases the half-life of thermal inactivation at 45°C from around 1 min to 2 min |
691424 |
1.20.1.1 | A319E/T101A |
increases the half-life of thermal inactivation at 45°C from around 1 min to 5 min |
691424 |
1.20.1.1 | A325V |
thermostability almost identical to that of the wild-type enzyme |
691424 |
1.20.1.1 | C336D |
increases solubility and activity, thermostability almost identical to that of the wild-type enzyme |
691424 |
1.20.1.1 | D13E |
increases solubility and activity, thermostability almost identical to that of the wild-type enzyme |
691424 |
1.20.1.1 | D13E/M26I/E175A/E332N/C336D |
mutant obtained by directed evolution, round 4 |
673144 |
1.20.1.1 | D13E/M26I/E175A/T181S/A308T/E332N/C336D |
mutant obtained by directed evolution, round 6, strong decrease in KM value for NADP compared to wild-type |
673144 |
1.20.1.1 | D13E/M26I/E175A/T181S/E332N/C336D |
mutant obtained by directed evolution, round 5 |
673144 |