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Results 1 - 10 of 98 > >>
EC Number
Amino acid exchange
Commentary
Reference
A146S
increases the half-life of thermal inactivation at 45°C from around 1 min to 8 min
A176R
strongly decreased Km for NADP+
A319E
increases the half-life of thermal inactivation at 45°C from around 1 min to 2 min
A319E/T101A
increases the half-life of thermal inactivation at 45°C from around 1 min to 5 min
A325V
thermostability almost identical to that of the wild-type enzyme
C336D
increases solubility and activity, thermostability almost identical to that of the wild-type enzyme
D13E
increases solubility and activity, thermostability almost identical to that of the wild-type enzyme
D13E/M26I/E175A/E332N/C336D
mutant obtained by directed evolution, round 4
D13E/M26I/E175A/T181S/A308T/E332N/C336D
mutant obtained by directed evolution, round 6, strong decrease in KM value for NADP compared to wild-type
D13E/M26I/E175A/T181S/E332N/C336D
mutant obtained by directed evolution, round 5
Results 1 - 10 of 98 > >>