EC Number   |
Protein Variants |
Reference |
|---|
   1.14.99.53 | H61A |
distance between the copper ion and the predicted active site residue are increased |
-, 766407 |
| 1.14.99.53 | Y142A |
distance between the copper ion and the predicted active site residue are increased |
-, 766407 |
| 1.14.99.53 | H1A |
hydrogen bonds between the copper ion and the predicted active site residues are similar to wild-type |
-, 766407 |
| 1.14.99.53 | W82Y/N85F/Q141W |
loss of chitin monooxygenase activity, 12.21% residual cellulose C1- and 2.1% residual C4-oxidizing activity, ratio C1:C4-activity is 5.06 |
745380 |
| 1.14.99.53 | W82Y/N85F/Y116F/Q141W |
loss of chitin oxidizinbg activity, 10.2% residual cellulose C1- and 1.6% residual C4-oxidizing activity, ratio C1:C4-activity is 5.78 |
745380 |
| 1.14.99.53 | D140A |
mutant shows moderately reduced activity and essentially unchanged oxidative regioselectivity |
-, 745380 |
| 1.14.99.53 | W82Y/N85F |
mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 10.9 |
745380 |
| 1.14.99.53 | W82Y/N85F/Y116F |
mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 14.7 |
745380 |
| 1.14.99.53 | W82Y |
mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 2.0 |
-, 745380 |
| 1.14.99.53 | W82Y/N85F/Q141W |
mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.1 |
745380 |
