EC Number   |
Protein Variants |
Reference |
|---|
   1.14.20.5 | more |
domain swapping experiments joining the N-terminus of flavanone 3beta-hydroxylase with the C-terminus of flavone synthase I and vice versa. Conversion of seven active site residues of flavanone 3beta-hydroxylase into the corresponding amino acids of flavone synthase I causes a nearly complete change in enzyme activity from flavanone 3beta-hydroxylase to flavone synthase I |
689577 |
| 1.14.20.5 | more |
gene DvFNS silencing by siRNA approach |
726253 |
| 1.14.20.5 | L311F/F146I/Y240P |
inactive mutant |
742512 |
| 1.14.20.5 | L311F/F146I/Y240P/A120M |
inactive mutant |
742512 |
| 1.14.20.5 | L311F/F146I/A120M |
the activity of the triple mutant falls to about 5% of the wild type enzyme |
742512 |
| 1.14.20.5 | L311F/A120M |
the double mutant catalyzes the formation of 2-hydroxynaringenin and apigenin, although the ratio of these two compounds iss lower than that generated by the wild type enzyme |
742512 |
| 1.14.20.5 | L311F/F146I |
the double mutant catalyzes the formation of 2-hydroxynaringenin and apigenin, although the ratio of these two compounds iss lower than that generated by the wild type enzyme |
742512 |
| 1.14.20.5 | L311F/Y240P |
the double mutant exhibits less flavone synthase activity than either the wild type or the Y240P single mutant enzymes |
742512 |
| 1.14.20.5 | F146I/A120M |
the enzyme activity of the double mutant is reduced to a level of about 10% of the activity of the wild type enzyme and the only product generated is apigenin |
742512 |
| 1.14.20.5 | Y240P/F146I |
the enzyme activity of the double mutant is reduced to a level of about 10% of the activity of the wild type enzyme and the only product generated is apigenin |
742512 |
