EC Number |
Protein Variants |
Reference |
---|
1.14.15.8 | A106T |
kcat/Km for conversion of 11-deoxycortisol is 3.17fold higher than wild-type value, 1.43fold increase in progesterone conversion |
693956 |
1.14.15.8 | A106T/Q189K/T399S/R409L |
kcat/Km for conversion of 11-deoxycortisol is 4.3fold higher than wild-type value, 1.13fold increase in progesterone conversion |
693956 |
1.14.15.8 | A106T/R409L |
kcat/Km for conversion of 11-deoxycortisol is 3fold higher than wild-type value, 1.43fold increase in progesterone conversion |
693956 |
1.14.15.8 | D153V/I214F |
kcat/Km for conversion of 11-deoxycortisol is 2.64fold higher than wild-type value, progesterone conversion is 93% of wild-type value |
693956 |
1.14.15.8 | D217V |
kcat/Km for conversion of 11-deoxycortisol is 2.68fold higher than wild-type value, 1.18fold increase in progesterone conversion |
693956 |
1.14.15.8 | F165I |
1.25fold increase in progesterone conversion |
693956 |
1.14.15.8 | more |
modelling of CYP106A2 and site-directed mutagenesis of the protein to check the accuracy of the computer-derived model of CYP106A2 |
696142 |
1.14.15.8 | S72A/V73I |
mutant does not show a better stability in the crystallization process than the wild-type protein |
696142 |
1.14.15.8 | A395L |
Vmax/Km for progesterone is 2fold lower than wild-type value |
697323 |
1.14.15.8 | E90V/D185G |
Vmax/Km for progesterone is 1.3fold higher than wild-type value |
697323 |