EC Number |
Protein Variants |
Reference |
---|
1.1.1.87 | R85V |
complete loss of activity with isocitrate, significant activity with 3-isopropylmalate, no effect on activity with homoisocitrate |
-, 656127 |
1.1.1.87 | more |
modulation of the broad substrate specificity of the trifunctional enzyme through site-directed mutagenesis, overview |
660918 |
1.1.1.87 | K206M |
site-directed mutagenesis, inactive mutant |
739958 |
1.1.1.87 | Y150F |
site-directed mutagenesis, inactive mutant |
739958 |
1.1.1.87 | D271N |
site-directed mutagenesis, mutation of a metal ion ligand and binding determinant for Mg2+, to N. The mutant enzyme shows a decrease of 520fold in V and V/Km_Mg2+, suggesting that the same step(s) limit the reaction at limiting and saturating MgHIc concentrations |
739958 |
1.1.1.87 | V135M |
site-directed mutagenesis, tetramer-to-dimer structural transition enhances the activity with isocitrate 1.6fold |
669109 |
1.1.1.87 | K206M |
site-directed mutagenesis, the active site mutant shows about 2400fold reduced activity compared to the wild-type enzyme, the Km for HIc does not change significantly |
696350 |
1.1.1.87 | Y150F |
site-directed mutagenesis, the active site mutant shows about 680fold reduced activity compared to the wild-type enzyme, the Km for HIc does not change significantly |
696350 |
1.1.1.87 | R87T |
site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate |
660918 |
1.1.1.87 | R87V |
site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate |
660918 |