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Results 1 - 10 of 12 > >>
EC Number
Amino acid exchange
Commentary
Reference
A80del
site-directed mutagenesis, the mutant shows altered substrate specificity preferring isocitrate to homoisocitrate, it is unable to oxidize 3-isopropylmalate, the specificity is similar to the enzyme from Thermus thermophilus
D271N
site-directed mutagenesis, mutation of a metal ion ligand and binding determinant for Mg2+, to N. The mutant enzyme shows a decrease of 520fold in V and V/Km_Mg2+, suggesting that the same step(s) limit the reaction at limiting and saturating MgHIc concentrations
K206M
site-directed mutagenesis, inactive mutant
K206M
site-directed mutagenesis, the active site mutant shows about 2400fold reduced activity compared to the wild-type enzyme, the Km for HIc does not change significantly
more
modulation of the broad substrate specificity of the trifunctional enzyme through site-directed mutagenesis, overview
more
trex mutant allele, T to C exchange at nucleotide 251. Midgestation lethal mutant which displays craniofacial, limb, and organ abnormalities due to insufficient retinoic acid signaling
R85V
complete loss of activity with isocitrate, significant activity with 3-isopropylmalate, no effect on activity with homoisocitrate
R87T
site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate
R87V
site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate
V135M
site-directed mutagenesis, tetramer-to-dimer structural transition enhances the activity with isocitrate 1.6fold
Results 1 - 10 of 12 > >>