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Results 1 - 6 of 6
EC Number
Amino acid exchange
Commentary
Reference
D194A
the mutation results in substantial catalytic deficiency (2.8% compared to the wild type enzyme)
H198A
inactive
H267A
inactive
H281A
inactive
H363A
the mutant with full activity is not able to grow on butanal-containing medium
P704L/H734R
the ethanol tolerant phenotype of Clostridium thermocellum is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE). The mutant displays a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity. The reduction in specific activity with respect to NADH is far greater (about 25fold less activity) than the increase with respect to NADPH. Although total ADH activity dropps by 25fold, ethanol production does not drop significantly between strains under these conditions
Results 1 - 6 of 6