EC Number   |
Protein Variants |
Reference  |
|---|
   1.1.1.53 | I15T |
in 20beta-HSD type A, cofactor binding is abolished |
287107 |
| 1.1.1.53 | Q27K |
in 20beta-HSD type B, cofactor binding is reduced |
287107 |
| 1.1.1.53 | T15I |
in 20beta-HSD type B, enzyme activity is restored |
287107 |
| 1.1.1.53 | I6T/V47M/T69K |
mutant enzyme with a maximal improvement in solubility, high catalytic activity as a NADP-dependent 3alpha,20beta-hydroxysteroid dehydrogenase |
-, 657157 |
| 1.1.1.53 | I6T/V47M/T69K/E142A |
pH optimum for reduction of progesterone shifts slightly from pH 6.0 to pH 6.5 compared to for mutant enzyme I6T/V47M/T69K, the oxidation of androsterone shows a dual pH optimum, one at pH 6.0-6.5 and the other at pH 10 |
-, 657157 |
| 1.1.1.53 | I6T/V47M/T69K/S140A |
both oxidation of androsterone and reduction of progesterone are completely lost |
-, 657157 |
| 1.1.1.53 | I6T/V47M/T69K/Y153F |
both oxidation of androsterone and reduction of progesterone are completely lost |
-, 657157 |
| 1.1.1.53 | V54L |
the mutation restricts the steroid alternative binding and enhances the 20alpha-hydroxysteroid dehydrogenase activity of the enzyme |
738928 |
