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Results 1 - 8 of 8
EC Number
Amino acid exchange
Commentary
Reference
I6T/V47M/T69K/S140A
both oxidation of androsterone and reduction of progesterone are completely lost
I6T/V47M/T69K/Y153F
both oxidation of androsterone and reduction of progesterone are completely lost
I15T
in 20beta-HSD type A, cofactor binding is abolished
Q27K
in 20beta-HSD type B, cofactor binding is reduced
T15I
in 20beta-HSD type B, enzyme activity is restored
I6T/V47M/T69K
mutant enzyme with a maximal improvement in solubility, high catalytic activity as a NADP-dependent 3alpha,20beta-hydroxysteroid dehydrogenase
I6T/V47M/T69K/E142A
pH optimum for reduction of progesterone shifts slightly from pH 6.0 to pH 6.5 compared to for mutant enzyme I6T/V47M/T69K, the oxidation of androsterone shows a dual pH optimum, one at pH 6.0-6.5 and the other at pH 10
V54L
the mutation restricts the steroid alternative binding and enhances the 20alpha-hydroxysteroid dehydrogenase activity of the enzyme
Results 1 - 8 of 8