EC Number |
Protein Variants |
Reference |
---|
1.1.1.346 | more |
construction of enzyme gene knockout mutant M-AKR, that shows decreased degradation activity with testosterone, estradiol, oestrone, and methyltestosterone compared to the wild-type enzyme. Compared to the wild-type, the mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone |
-, 742259 |
1.1.1.346 | more |
establishment of an efficient process for 2,5-DKG reductase production that also satisfies food safety requirements. Food grade variants of the Lactobacillales based expression systems pSIP (Lactobacillus plantarum) and NICE (Lactococcus lactis) are evaluated with regard to their effictiveness to produce 2,5-DKG reductase from Corynebacterium glutamicum, overview. Lactobacillus plantarum/pSIP609 is an interesting alternative to Escherichia coli expression systems for industrial 2,5-DKG reductase production. Highest production levels of 2,5-DKG reductase are obtained with the system Lactobacillus plantarum/pSIP609, resulting in 104 U/l without pH regulation and 262 U/l with pH control at pH 6.5 |
-, 741575 |
1.1.1.346 | F22Y/K232G/R235T/R238H/A272G 420 |
mutant with decreased kcat value for NADPH compared to the wild type enzyme |
440311 |
1.1.1.346 | F22Y/K232G/R238H/A272G |
mutant with decreased kcat value for NADPH compared to the wild type enzyme |
440311 |
1.1.1.346 | K232G/R238H |
mutant with decreased kcat value for NADPH compared to the wild type enzyme |
440311 |
1.1.1.346 | F22Y/K232G/R235G/R238H/A272G |
mutant with wild type kcat value for NADPH |
440311 |
1.1.1.346 | F22Y/A272G |
substrate-binding pocket double mutant with decreased kcat value for NADPH compared to the wild type enzyme |
440311 |
1.1.1.346 | F22Y/K232G/R238H/A272G |
the mutant exhibits activity with NADH that is more than 2 orders of magnitude higher than that of the wild type enzyme and retains a high level of activity with NADPH |
440311 |
1.1.1.346 | K233G |
the mutant shows decreased NADPH activity and increased NADH activity compared to the wild type enzyme |
440310 |
1.1.1.346 | K233M |
the mutant shows decreased NADPH activity and increased NADH activity compared to the wild type enzyme |
440310 |