EC Number   |
Protein Variants  |
Reference |
|---|
    1.1.1.248 | D107A |
increase in Km, 2fold decrease in kcat |
698937 |
| 1.1.1.248 | D107A |
the mutant shows altered kinetics compared to the wild-type enzyme |
725460 |
| 1.1.1.248 | F104A |
increase in kcat |
698937 |
| 1.1.1.248 | F104A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
689571 |
| 1.1.1.248 | F104A |
the mutant shows altered kinetics compared to the wild-type enzyme |
725460 |
| 1.1.1.248 | F104A/I275A |
shows no substrate inhibition accompanied by a weak affinity for productive salutaridine binding, kcat is almost 2fold higher compared with the wild-type |
698937 |
| 1.1.1.248 | F104A/I275A |
substitution of Phe104 in the substrate-binding pocket, and Ile275 under the flap domain, the mutant shows altered kinetics compared to the wild-type enzyme |
725460 |
| 1.1.1.248 | I275A |
increase in Km, shows an increase of velocity by a factor of 2.3 |
698937 |
| 1.1.1.248 | I275A |
the mutant shows altered kinetics compared to the wild-type enzyme |
725460 |
| 1.1.1.248 | I275V |
the mutant shows altered kinetics compared to the wild-type enzyme |
725460 |
