EC Number   |
Protein Variants |
Reference  |
|---|
    1.1.1.22 | more |
comparison of sequence homologies with bacterial enzymes |
286306 |
| 1.1.1.22 | more |
overexpression of enzyme plus transformation of gene cluster for K5 polysaccharide production, 15fold increase in enzyme activity, decrease in K5 polysaccharide formation |
654528 |
| 1.1.1.22 | C260A |
no oxidation of UDP-glucose to glucuronic acid, but capable of both reducing the aldehyde intermediate and oxidizing the hydrated form of the aldehyde intermediate, protein is expressed in inclusion bodies |
655477 |
| 1.1.1.22 | E141Q |
kcat-value 10fold lower than wild-type |
655477 |
| 1.1.1.22 | E145Q |
kcat-value 10fold lower than wild-type |
655477 |
| 1.1.1.22 | T118A |
160fold reduction of kcat value |
655477 |
| 1.1.1.22 | C276S |
no enzymic activity, affinity for NAD+ similar to wild-type, retains predominantly hexameric structure |
656220 |
| 1.1.1.22 | K279A |
no enzymic activity, affinity for NAD+ similar to wild-type, almost exclusively found as dimer |
656220 |
| 1.1.1.22 | G13E |
normal expression and stability of mutant, no enzymic activity, no photoaffinity labeling with nicotinamide 2-azidoadenosine dinucleotide |
656240 |
| 1.1.1.22 | C276E |
activity is not measurable at pH 8.7, 22°C |
667848 |
