EC Number   |
Protein Variants |
Reference |
|---|
   4.1.99.5 | I127G |
site-directed mutagenesis, increased activity compared to wild-type |
-, 747353 |
| 4.1.99.5 | I127G/A48G |
site-directed mutagenesis, increased activity compared to wild-type |
-, 747353 |
| 4.1.99.5 | I24Y |
site-directed mutagenesis, the mutant shows increased activity with n-heptanal compared to the wild-type enzyme |
-, 747351 |
| 4.1.99.5 | I27F |
site-directed mutagenesis, the mutant shows increased activity with n-decanal and n-dodecanal compared to the wild-type enzyme |
747351 |
| 4.1.99.5 | L146T |
site-directed mutagenesis, altered activity compared to wild-type |
747409 |
| 4.1.99.5 | L148R |
site-directed mutagenesis, altered activity compared to wild-type |
747409 |
| 4.1.99.5 | L194A |
site-directed mutagenesis, the mutant has kinetic properties very similar to the wild-type enzyme |
-, 746597 |
| 4.1.99.5 | L198F |
site-directed mutagenesis, the mutant shows increased activity with C7-10 aldehydescompared to the wild-type enzyme |
-, 747351 |
| 4.1.99.5 | M193Y |
site-directed mutagenesis, the mutant shows increased activity with C6-10 aldehydes compared to the wild-type enzyme |
747351 |
| 4.1.99.5 | more |
alteration of the enzyme's substrate specificity by engineering of active site residues involved in substrate binding, residues V41 and A134, adjacent to the C9 position of the ligand, might influence fatty acid binding, overview |
-, 727312 |
