EC Number |
Protein Variants |
Reference |
---|
3.5.99.7 | more |
construction of a disruption mutant deficient of enzyme activity by insertion of a tetracycline resistance gene into the the coding region of the chromosomal copy of the ACC deaminase gene |
-, 679006 |
3.5.99.7 | more |
construction of a mutant Pseudomonas migulae strain 8R6 lacking the ACC deaminase activity. The bacterial wild-type strain 8R and its mutant lacking the ACC deaminase activity do not promote the growth of either the infected or uninfected plants |
-, 757954 |
3.5.99.7 | more |
gene acdS expressing mutant strains are generated from ACCD negative psychrotolerant bacterial strains Flavobacterium sp. OR306 and Pseudomonas frederiksbergensis OS211, isolated from agricultural soil during late winter. After transformation with plasmid pRKACC-acdS, both the strains are able to exhibit ACCD activity in vitro. On exposing tomato plants inoculated with both acdS expressing and wild-type bacteria to one week of chilling treatment at 12/10°C, stress ethylene, ACC accumulation and ACO activity, which are markers of ethylene stress, are significantly reduced in plants inoculated with the acdS gene transformed mutants. In case of plants inoculated with strain OS211-acdS, ethylene emission, ACC accumulation and ACO activity are significantly reduced by 52%, 75.9%, and 23.2%, respectively, compared to uninoculated control plants. Expression analysis of cold-induced LeCBF1 and LeCBF3 genes shows that these genes in the plants are significantly induced by the acdS transformed mutant bacteria in addition to observed reduced expression of ethylene-responsive transcription factor 13 (ETF-13) and ACO genes |
-, 758070 |
3.5.99.7 | more |
gene-disruption mutant, decreased nodulation efficiency with Pisum sativum |
648267 |
3.5.99.7 | more |
generation of a Streptomyces sp. GMKU 336 ACCD-deficient gene disruption mutant, the mutant strain is catalytically inactive |
758431 |
3.5.99.7 | more |
random mutagenesis generating a library of mutants for the the elucidation of structure-function relationships |
34580 |
3.5.99.7 | Y268F |
site-directed mutagesis, mutant kinetics compared to the wild-type, overview |
-, 718894 |
3.5.99.7 | Y294F |
site-directed mutagesis, mutant kinetics compared to the wild-type, overview |
-, 718894 |
3.5.99.7 | Y295F |
crystal structure in complex with substrate |
648271 |