EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.9 | K63R |
site directed mutagenesis |
716743 |
| 3.4.21.9 | K99A |
no cleavage of trypsinogen or Gly-(Asp)4-Lys-beta-naphthylamide and reduced rate of inhibition by Val-(Asp)4-Lys-chloromethane |
95577 |
| 3.4.21.9 | more |
even mild protein surface supercharging by mutagenesis can have pronounced effects on protein solubility and stability, overview |
718352 |
| 3.4.21.9 | more |
improved efficiency of fusion proteins cleavage by enteropeptidase by substitution of the Lys residue by Arg in specific cleavage sequence (Asp)4-Lys |
718359 |
| 3.4.21.9 | more |
in order to produce more site-specific enterokinase light chain, EKL, several mutants are generated with substitutions at Tyr174 and Lys99 using the protein disulfide isomerase fusion system. Substitution of Tyr174 by basic residues confers higher specificity on EKL. Mutant enzyme substrate specificity and modeling, overview |
717409 |
| 3.4.21.9 | N6D/G21D/G22D/N141D/K209E |
site-directed mutagenesis, the mutations lead to supercharging of the protein surface leading to 100fold increased protein solubility |
718352 |
| 3.4.21.9 | N6D/G21D/G22D/N142D/K210E/C112S |
supercharged variant with increased solubility more than 100fold, used for crystallization |
732888 |
| 3.4.21.9 | P193A |
site directed mutagenesis |
716743 |
| 3.4.21.9 | P193E |
site directed mutagenesis |
716743 |
| 3.4.21.9 | R213L |
the mutant shows increased activity compared to the wild type enzyme |
732855 |
