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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123E351S the endo-glycoceramidase II nucleophile mutant E351S has glycosynthase activity 714723
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123E351W mutant glycosidase with no glycosynthase activity 695545
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123E351Y mutant glycosidase with no glycosynthase activity 695545
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123K61A no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide -, 751102
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123N213A no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide -, 751102
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123N265A no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide. Hydrolysis of beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide is 2.1% compared to wild-type enzyme 751102
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123Q298A no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide -, 751102
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123S63G the mutant enzyme shows a broader substrate spectrum and significantly increased activity toward bulky substrates 751102
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123W365A no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide 751102
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.123Y302A no hydrolysis of beta-D-Gal-(1->3)-beta-D-GalNAc-(1->4)-(alpha-Neu5Ac-(2->3))-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide and beta-D-Gal-(1->4)-beta-D-Glc-(1<->1')-ceramide 751102
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