EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.84 | A199S/S287G/A328W/Y332G/L286I |
the mutant has a 242fold improved catalytic efficiency against (-)-cocaine compared to the wild type enzyme |
714226 |
| 3.1.1.84 | A328W/Y332A |
the mutant has a 9.4fold improved catalytic efficiency against (-)-cocaine compared to the wild type enzyme |
714226 |
| 3.1.1.84 | A328W/Y332G |
the mutant has a 15fold improved catalytic efficiency against (-)-cocaine compared to the wild type enzyme |
714226 |
| 3.1.1.84 | D259N |
mutation results in more than 1500fold decrease in kcat |
650108 |
| 3.1.1.84 | F227A/S287G/A328W/Y332M |
the mutant has a 34fold improved catalytic efficiency against (-)-cocaine compared to the wild type enzyme |
714226 |
| 3.1.1.84 | F261A |
mutant catalyzed the hydrolysis of cocaine with a 29fold lower kcat and 15fold higher KM |
650108 |
| 3.1.1.84 | F408A |
mutant has 8fold increased KM and more than 100fold decrease in kcat |
650108 |
| 3.1.1.84 | G173Q |
kcat and Km-value for cocaine is similar to wild-type value, half-life is increased 7fold compared to wild-type enzyme |
694267 |
| 3.1.1.84 | G173Q |
the mutant does not have any deleterious effects on the catalytic efficiency |
-, 716824 |
| 3.1.1.84 | G173Q/L169K |
the mutant has a half-life of 370 min and 2.9 days at 37°C |
716281 |
