EC Number   |
Protein Variants |
Reference |
|---|
    2.7.2.8 | R24E |
site-directed mutagenesis, the mutant shows reduced Vmax , the mutation results in increased affinity of NAGK for arginine |
692856 |
| 2.7.2.8 | R386K |
site-directed mutagenesis, inactive mutant |
735596 |
| 2.7.2.8 | R394K |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
-, 735596 |
| 2.7.2.8 | R66K |
substantial although diminished activity |
659704 |
| 2.7.2.8 | S387A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
735596 |
| 2.7.2.8 | S395A |
site-directed mutagenesis, the mutant shows slightly reduced activity compared to the wild-type enzyme |
-, 735596 |
| 2.7.2.8 | W23A |
Km (N-acetyl-L-glutamate) similar to wild-type, kcat similar to wild-type, IC50 (L-arginine) 4.5fold increased compared to wild-type |
722087 |
| 2.7.2.8 | Y21A |
site-directed mutagenesis, the mutant shows reduced Vmax and altered Km for the substrates |
692856 |
| 2.7.2.8 | Y397F |
site-directed mutagenesis, inactive mutant |
735596 |
| 2.7.2.8 | Y405F |
site-directed mutagenesis, inactive mutant |
-, 735596 |
