EC Number |
Protein Variants |
Reference |
---|
2.3.1.12 | G610C |
500fold reduced enzymatic activity |
486228 |
2.3.1.12 | H602C |
inactive |
736448 |
2.3.1.12 | I176A |
mutation in the inner lipoyl-bearing domain (L2), pyruvate dehydrogenase kinase 2 binding: not measurable |
672273 |
2.3.1.12 | I229A |
ability to be post-translationally lipoylated remains |
486229 |
2.3.1.12 | I229A/F231A |
ability to be post-translationally lipoylated remains, far-UV CD spectrum differs from wild-type enzyme |
486229 |
2.3.1.12 | K136A |
site-directed mutagenesis, thermodynamics and kinetics in comparison to the wild-type enzyme |
661632 |
2.3.1.12 | K153A |
site-directed mutagenesis, thermodynamics and kinetics in comparison to the wild-type enzyme |
661632 |
2.3.1.12 | K173A |
mutation in the inner lipoyl-bearing domain (L2), pyruvate dehydrogenase kinase 2 binding: not measurable |
672273 |
2.3.1.12 | K191E |
the mutant shows 50% activity compared to the wild type enzyme |
725521 |
2.3.1.12 | K191R |
the mutant shows 79% activity compared to the wild type enzyme |
725521 |