EC Number   |
Protein Variants |
Reference  |
|---|
    1.3.98.1 | R50E |
little changes in activity |
656148 |
| 1.3.98.1 | R57A |
increase in activity |
656148 |
| 1.3.98.1 | S129A |
decrease in activtiy against fumarate |
656148 |
| 1.3.98.1 | H185A |
4fold increase in KM-value of CoQD, 50% increase in KM-value of L-dihydroorotate |
656285 |
| 1.3.98.1 | R265A |
2fold increase in KM-value of CoQD, 15% increase in KM-value of L-dihydroorotate |
656285 |
| 1.3.98.1 | E206/K296E |
conversion of intermolecular salt bridge, mutant is fully active in concentrated solutions and dissociates into monomers upon dilution like wild-type enzyme |
657291 |
| 1.3.98.1 | E206A |
disturbance of intermolecular salt bridge, mutant retains almost complete activity |
657291 |
| 1.3.98.1 | E206K |
disturbance of intermolecular salt bridge, mutant activity is severely impaired |
657291 |
| 1.3.98.1 | K296A |
disturbance of intermolecular salt bridge, mutant retains almost complete activity |
657291 |
| 1.3.98.1 | K296E |
disturbance of intermolecular salt bridge, mutant activity is severely impaired |
657291 |
