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<< < Results 11 - 20 of 98 > >>
EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1D13E/M26I/E332N/C336D mutant obtained by directed evolution, round 3 673144
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336D thermostable mutant TS-PTDH which contains 12 mutations that result in considerably increased thermostability with minimal change in activity, and four additional mutations that increase its activity. Mutation E175A leads to relaxation of cofactor specificity 726982
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1D79A significant differences in its kinetic constants compared to the wild-type enzyme. 2600fold decrease in catalytic efficiency. Pre-steady-state rates are approximately the same as the steady-state rates -, 690884
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1D79N has kinetic parameters more similar to those of wild-type 690884
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1E130K increases the half-life of thermal inactivation at 45°C from around 1 min to 12.5 min 691424
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1E130Q increases the half-life of thermal inactivation at 45°C from around 1 min to 7 min 691424
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1E130R increases the half-life of thermal inactivation at 45°C from around 1 min to 9 min 691424
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1E175A increases solubility and activity, thermostability almost identical to that of the wild-type enzyme 691424
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1E175A mutant with significantly increased kcat value for NADP+ 673144
Display the word mapDisplay the reaction diagram Show all sequences 1.20.1.1E175A strongly decreased Km for NADP+ 657956
<< < Results 11 - 20 of 98 > >>