EC Number   |
Protein Variants |
Reference |
|---|
   1.14.11.6 | H214A |
site-directed mutagenesis, the mutant shows highly reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme |
745921 |
| 1.14.11.6 | H239A |
mutation abrogates the ability of JBP1 to complement JBP1 null mutants |
689246 |
| 1.14.11.6 | H271A |
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme |
745921 |
| 1.14.11.6 | H391A |
site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2, expression of the TH mutant H319A fails to stimulate base J formation |
700501 |
| 1.14.11.6 | H414A |
site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect |
700084 |
| 1.14.11.6 | H441A |
site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain |
700501 |
| 1.14.11.6 | H465A |
site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect |
700084 |
| 1.14.11.6 | L192A |
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme |
745921 |
| 1.14.11.6 | L223A |
site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme |
745921 |
| 1.14.11.6 | more |
construction of a C-terminally truncated enzyme mutant NcT7H, comprising residues 1-299 |
745921 |
