F107Y
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H117A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H51A
site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme
H55A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H93A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
H93N
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
L87A
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
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construction of enzyme-deficient strains by gene ectC inframe deletion though the splicing-by-overlap-extension PCR technique
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expression in transgenic Nicotiana tabacum BY2 cells increases the osmotolerance and growth rate of the cells under hyperosmotic conditions
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structure-guided site-directed mutagenesis is used targeting amino acid residues that are evolutionarily highly conserved among the extended EctC protein family, including those forming the presumptive iron-binding site