EC Number   |
Protein Variants |
Reference  |
|---|
   3.1.3.56 | R220A |
site-directed mutagenesis, mutation in the P-loop/alpha4-helix, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview |
751120 |
| 3.1.3.56 | R252A |
site-directed mutagenesis, mutation in the alpha4-alpha5-loop, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview |
751120 |
| 3.1.3.56 | W234A |
site-directed mutagenesis, mutation in the bulky and hydrophobic loop, the mutant is only active with 5-diphosphoinositol 1,2,3,4,6-pentakisphosphate |
751120 |
| 3.1.3.56 | W281A |
site-directed mutagenesis, mutation in the bulky and hydrophobic loop, inactive mutant |
751120 |
| 3.1.3.56 | more |
T-DNA insertional mutants for 5PTase1 (5ptase1-1 and 5ptase1-2, containing a T-DNA insertion within the fifth and fourth introns) and 5PTase2 (5ptase2-1 which contains a T-DNA insertion in the seventh intron) are identified and characterized and a At5PTase1-1/At5PTase2-1 double mutant is produced: grown in dark, seeds from mutants germinate faster than wild-type seeds and the mutant seedlings have longer hypocotyls than wild-type seedlings. Seeds from these mutant lines demonstrate an increase in sensitivity to abscisic acid. These changes in early seedling growth are accompanied by mass increases in Ins(1,4,5)P3, but not by changes in endogenous ABA content. By labeling the endogenous myoinositol pool in 5ptase1 and 5ptase2 mutants, an increases in Ins(1,4,5)P3 and a decrease in PtdIns, PtdIns(4)P, and phosphatidylinositol (4,5)bisphosphate is detected |
682411 |
| 3.1.3.56 | more |
T-DNA insertional mutants for 5PTase1 (5ptase1-1 and 5ptase1-2, containing a T-DNA insertion within the fifth and fourth introns) and 5PTase2 (5ptase2-1 which contains a T-DNA insertion in the seventh intron) are identified and characterized and a At5PTase1-1/At5PTase2-1 double mutant is produced: grown in dark, seeds from mutants germinate faster than wildtype seeds and the mutant seedlings have longer hypocotyls than wild-type seedlings. Seeds from these mutant lines demonstrate an increase in sensitivity to abscisic acid. These changes in early seedling growth are accompanied by mass increases in Ins(1,4,5)P3, but not by changes in endogenous ABA content. By labeling the endogenous myoinositol pool in 5ptase1 and 5ptase2 mutants, an increases in Ins(1,4,5)P3 and a decrease in PtdIns, PtdIns(4)P, and phosphatidylinositol (4,5)bisphosphate is detected |
682411 |
| 3.1.3.56 | more |
construction of transgenic Arabidopsis thaliana plants expressing the human enzyme, the transgenic plants show no altered growth and life cycle phenotype although the basal level of inositol 1,4,5-trisphosphate is reduced by 90% compared to wild-type plants, but the plants are insensitive to gravitropic stimulation and show no increased InsP3 levels in inflorescence stem under gravistimulation in contrast to wild-type plants, bending responses, overview |
666645 |
| 3.1.3.56 | E597Q |
site-directed mutagenesis, the mutant enzyme shows an over 140fold increased catalytic efficiency and a 2.4fold reduced affinity for Mg2+ compared to the wild-type enzyme |
665575 |
| 3.1.3.56 | D838G |
no activity |
646402 |
| 3.1.3.56 | D232A |
no activity |
646400 |
