EC Number   |
Protein Variants |
Reference |
|---|
   3.1.3.56 | more |
T-DNA insertional mutants for 5PTase1 (5ptase1-1 and 5ptase1-2, containing a T-DNA insertion within the fifth and fourth introns) and 5PTase2 (5ptase2-1 which contains a T-DNA insertion in the seventh intron) are identified and characterized and a At5PTase1-1/At5PTase2-1 double mutant is produced: grown in dark, seeds from mutants germinate faster than wild-type seeds and the mutant seedlings have longer hypocotyls than wild-type seedlings. Seeds from these mutant lines demonstrate an increase in sensitivity to abscisic acid. These changes in early seedling growth are accompanied by mass increases in Ins(1,4,5)P3, but not by changes in endogenous ABA content. By labeling the endogenous myoinositol pool in 5ptase1 and 5ptase2 mutants, an increases in Ins(1,4,5)P3 and a decrease in PtdIns, PtdIns(4)P, and phosphatidylinositol (4,5)bisphosphate is detected |
682411 |
| 3.1.3.56 | more |
T-DNA insertional mutants for 5PTase1 (5ptase1-1 and 5ptase1-2, containing a T-DNA insertion within the fifth and fourth introns) and 5PTase2 (5ptase2-1 which contains a T-DNA insertion in the seventh intron) are identified and characterized and a At5PTase1-1/At5PTase2-1 double mutant is produced: grown in dark, seeds from mutants germinate faster than wildtype seeds and the mutant seedlings have longer hypocotyls than wild-type seedlings. Seeds from these mutant lines demonstrate an increase in sensitivity to abscisic acid. These changes in early seedling growth are accompanied by mass increases in Ins(1,4,5)P3, but not by changes in endogenous ABA content. By labeling the endogenous myoinositol pool in 5ptase1 and 5ptase2 mutants, an increases in Ins(1,4,5)P3 and a decrease in PtdIns, PtdIns(4)P, and phosphatidylinositol (4,5)bisphosphate is detected |
682411 |
| 3.1.3.56 | N183A |
site-directed mutagenesis, mutation in the Flex-loop, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview |
-, 751120 |
| 3.1.3.56 | N18A |
no activity |
646400 |
| 3.1.3.56 | N215A |
site-directed mutagenesis, mutation in the P-loop, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview |
-, 751120 |
| 3.1.3.56 | P387A |
traces of activity |
646400 |
| 3.1.3.56 | R216A |
site-directed mutagenesis, mutation in the P-loop, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview |
751120 |
| 3.1.3.56 | R220A |
site-directed mutagenesis, mutation in the P-loop/alpha4-helix, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview |
751120 |
| 3.1.3.56 | R252A |
site-directed mutagenesis, mutation in the alpha4-alpha5-loop, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview |
751120 |
| 3.1.3.56 | R343A |
increased Km and dramatic loss of activity |
646396 |
