EC Number   |
Protein Variants |
Reference |
|---|
   3.1.21.4 | D149A |
no detectable activity in ssDNA nicking, mutant R1 subunit/wild-type R2 subunit |
751757 |
| 3.1.21.4 | D226A |
no detectable activity in ssDNA nicking, mutant R2 subunit/wild-type R1 subunit |
751757 |
| 3.1.21.4 | D299A |
no catalytic activity, mutant like wild-type remains in dimeric form |
683465 |
| 3.1.21.4 | D317A |
84% of DNA binding compared to wild-type |
684042 |
| 3.1.21.4 | D321A |
119% of DNA binding compared to wild-type |
684042 |
| 3.1.21.4 | D328A |
76% of DNA binding compared to wild-type |
684042 |
| 3.1.21.4 | D329A |
123% of DNA binding compared to wild-type |
684042 |
| 3.1.21.4 | D329A |
less than 0.01% residual activity, mutant like wild-type remains in dimeric form |
683465 |
| 3.1.21.4 | DELTA362-465 |
nicking variant of BsaI, nicking occurs on top strand |
652938 |
| 3.1.21.4 | DELTA440-544 |
nicking variant of BsaI, nicking occurs on bottom strand |
652938 |
