EC Number   |
IC50 Value |
IC50 Value Maximum |
Inhibitor |
Reference |
|---|
   1.3.1.20 | 0.00097 |
- |
4-hydroxyacetophenone |
determined in the camphorquinone reduction, inhibitor is uncompetitive with respect to camphorquinone |
684150 |
| 1.3.1.20 | 0.00097 |
- |
4-hydroxyacetophenone |
the hydroxyl group of 4-hydroxyacetophenone forms hydrogen bond interactions with the side-chain oxygen and the main chain nitrogen of Asp280, the benzene ring and methyl group make a number of hydrophobic contacts with Phe279, Leu158 and Leu177 |
701119 |
| 1.3.1.20 | 0.00097 |
- |
4-hydroxyacetophenone |
wild type enzyme |
689972 |
| 1.3.1.20 | 0.00097 |
- |
4-hydroxyacetophenone |
wild type enzyme, in 25 mM Tris-HCl pH 7.5, at 25°C |
684150 |
| 1.3.1.20 | 0.006 |
- |
4-hydroxyacetophenone |
mutant enzyme W254Y, in 25 mM Tris-HCl pH 7.5, at 25°C |
684150 |
| 1.3.1.20 | 0.006 |
- |
4-hydroxyacetophenone |
mutant W254Y |
684150 |
| 1.3.1.20 | 0.037 |
- |
isoascorbic acid |
wild type enzyme, in 25 mM Tris-HCl pH 7.5, at 25°C |
684150 |
| 1.3.1.20 | 0.038 |
- |
isoascorbic acid |
determined in the camphorquinone reduction, inhibitor is uncompetitive with respect to camphorquinone |
684150 |
| 1.3.1.20 | 0.038 |
- |
isoascorbic acid |
mutant enzyme W254Y, in 25 mM Tris-HCl pH 7.5, at 25°C |
684150 |
| 1.3.1.20 | 0.038 |
- |
isoascorbic acid |
mutant W254Y |
684150 |
