EC Number   |
|---|
   7.4.2.6 | OppA in the open-and closed-liganded conformation, complexed with bradykinin, the nonapeptide RPPGFSPFR, hanging drop vapour diffusion method, 0.001 ml of protein solution are mixed with 10 mg/ml ligand-free OppA, 9 mM Na-MES, pH 6.0, 9 mM NaCl and 1 mM peptide, is mixed with 0.001 ml of reservoir solution containing 0.2 M NaCl, 0.1 M Na-HEPES, pH 7.0, and 20% PEG 6000, 12 weeks at room temperature, X-ray diffraction structure determination and analysis at 1.3 and 2.5 A resolution, respectively |
| 7.4.2.6 | purified soluble form of AppA in complex with a nonapeptide, hanging drop vapour diffusion method, unlabeled enzyme from 12-14% PEG 8000, 0.1-0.2 M zinc acetate, 0.1 M MES, pH 6.5, or Tris-HCl, pH 7.5, selenium-labeled enzyme from 16-18% PEG 8000, 0.1 M zinc acetate, and 0.1 M MES, pH 6.5, or Tris-HCl, pH 7.5, cryoprotection by 25% v/v glycerol, X-ray diffraction structure determination and analysis at 2.3-1.6 A resolution, selenium-based anomalous scattering and molecular replacement methods |
| 7.4.2.6 | sitting drop vapor diffusion method, using 100 mM HEPES pH 7.5, 1.0 M trisodium citrate |
