7.3.2.6 | purified recombinant component TupA, hanging drop vapor diffusion method, mixing of 0.002 ml of 7.5 mg/ml protein in 5 mM Tris-HCl, pH 7.5, with 0.001 ml of reservoir solution containing 0.2 M magnesium chloride, 0.1 M HEPES, pH 7.5, and 30% w/v PEG 3350, 20°C, 4 days, X-ray diffraction structure determination and analysis at 1.4 A resolution, molecular replacement |