EC Number   |
Reference |
|---|
   6.3.4.18 | hanging drop vapor diffusion method, using 0.1 M bis-Tris pH 6.5, 28% (w/v) polyethylene glycol monomethyl ether 2000 |
726578 |
| 6.3.4.18 | hanging drop vapour diffusion of native enzyme, crystals of the native enzyme belong to space group C222(1) with unit cell dimensions of a = 93.4 A, b = 95.2 A and c = 120.6 A. Crystals of the enzyme/MgADP complex are grown by batch methods at room temperature with poly(ethylene glycol)8000 as precipitant. The crystals belong to the space group P1 with unit cell dimensions of a = 60.6 A, b = 92.1 A, c = 102.6 A, alpha = 66.1°, beta = 82.7° and gamma = 81.8° |
661054 |
| 6.3.4.18 | structures with various combinations of ATP, ADP, Mg2+, hydrogencarbonate and aminoimidazole ribonucleotide in the active site. The binding involves interactions with the conserved residues Arg272, His274 and Lys348. The formation of a carboxyphosphate intermediate through ATP phosphoryl transfer is proposed, followed by carboxylation of AIR to give the product |
743938 |
