EC Number   |
|---|
    6.3.3.2 | cocrystallization with folinate, ATP and Mg2+, sitting drop vapour diffusion method using 25% polyethylene glycol 3350, 100 mM bis-Tris pH 5.5 |
| 6.3.3.2 | purified recombinant enzyme complexed with ADP, phosphate, and 5-formyltetrahydrofolate, hanging drop vapour diffusion method, 20°C, 30 mg/ml protein with 20% PEG 4000, 5 mM ATP, and 10 mM 5-formylTHF, flash freezing of all crystals in 20% PEG 4000 and 30% ethylene glycol, X-ray diffraction structure determination and analysis at 2.5 A resolution, crystallization of the enzyme with ATP analogues ATP-gamma-S, AMP-PNP, or AMP-PCP is not successful |
| 6.3.3.2 | purified recombinant His-tagged wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 30 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 1 mM EDTA, 1 mM DTT, at room temperature, 30 mg/ml selenomethionine-labeled enzyme in 0.2 M ammonium sulfate, 0.1 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, at 20°C, flash freezing of all crystals in a solution containing 0.17 M ammonium sulfate, 0.085 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, X-ray diffraction structure determination and analysis at 2.2 A resolution |
| 6.3.3.2 | purified recombinant wild-type MTHFS in a binary complex with ADP, with the N5-iminium phosphate reaction intermediate, or as an enzyme-product complex, hanging drop method at 16°C, 600 nl protein solution, with or without 5 mM 5-formyltetrahydrofolate and 5 mM ATP, are equilibrated against 0.05 ml of reservoir solution containing 100 mM HEPES, pH 6.6, 20 mM MgCl2, 20 mM NiCl2, and 20% w/v PEG 3350, 2 days, X-ray diffraction structure determination and analysis |
