EC Number   |
|---|
    5.5.1.2 | - |
| 5.5.1.2 | ammonium sulfate precipitation, Semet protein, determination of structure at 3.0 A resolution |
| 5.5.1.2 | characterization of two crystal forms |
| 5.5.1.2 | hanging drop vapour diffusion method with 12-18% PEG8000, 5 mM dithiothreitol, and 40 mM sodium phosphate (pH 7.3) |
| 5.5.1.2 | hanging-drop vapor-diffusion method, 2.6 A resolution, symmetric of hexagonal space group P6(5) with unit cell parameters: a = b = 231.52 A, c = 78.46 A, alpha = beta = 90°, gamma = 120°. There are four PpCMLE molecules, forming a homotetramer, in the asymmetric unit |
| 5.5.1.2 | recombinant enzyme at 2.2 A resolution. C-terminal domain present in all other members of fumarase II family is missing and enzyme contains an Arg residue as opposed to Trp in the active site. Activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization |
| 5.5.1.2 | SeMet protein, X-ray structure at 2.5 A is a seven-bladed beta propeller |
