EC Number   |
|---|
    5.5.1.1 | - |
| 5.5.1.1 | crystal structure at 1.85 A resolution |
| 5.5.1.1 | crystal structure at 3 A resolution |
| 5.5.1.1 | crystal structure at 6.5 A resolution |
| 5.5.1.1 | hanging drop vapor diffusion method, packing of the octameric enzyme in the crystal form is unusual, because the asymmetric unit contains three subunits |
| 5.5.1.1 | in complex with Mg2+, hanging drop vapor diffusion method, using 10% (v/v) 2-propanol, 0.1 M MES, pH 6.0, and 0.2 M Ca(OAc)2, or in complex with Mg2+ and cis,cis-muconate, hanging drop vapor diffusion method, using 1.0 M (NH4)2SO4, 0.1 M HEPES, pH 7.0, and 0.5% (w/v) PEG 8000 |
| 5.5.1.1 | in complex with Mg2+, hanging drop vapor diffusion method, using 20% (v/v) PEG 1000, 0.1 M cacodylate, pH 6.5, and 0.2 M MgCl2, or in complex with Mg2+ and cis,cis-muconate, hanging drop vapor diffusion method, using 2.0 M (NH4)2SO4, 0.1 M MES, pH 6.0, and 5% (v/v) isopropanol |
| 5.5.1.1 | muatnt enzyme F329I and I54V |
| 5.5.1.1 | sitting-drop vapor diffusion method |
| 5.5.1.1 | the crystallographic structure of the chloromuconate cycloisomerase from Rhodococcus opacus is determined at 2.5 A of resolution. Results highlight that a histidine, located in a loop that closes the active site cavity upon the binding of the substrate, could be related to the dehalogenation inability of Rho-2-CMCI |
