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EC Number
Crystallization (Commentary)
Reference
5.4.99.64
crystal structure in complex with coenzyme B12 and the substrates (S)-3-hydroxybutyryl- and 2-hydroxyisobutyryl-CoA in alternative binding. subunit HcmA residues IleA90 and AspA117. AspA117 determine the orientation of the hydroxyl group of the acyl-CoA esters by H-bond formation, thus determining stereospecificity of catalysis
738677
5.4.99.64
crystal structure of the enzyme in complex with coenzyme B12 and the substrates (S)-3-hydroxybutyryl- and 2-hydroxyisobutyryl-CoA in alternative binding. Crystals are grown in 16% PEG 3350, 0.1 M Tris,pH 7.5, by the hanging-drop vapor diffusion method at 4°C
738677
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